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. 2020 Dec 5;11(12):1035. doi: 10.1038/s41419-020-03241-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Western blot analysis of lysates of Med8A-R and STAT3^−/− cells for STAT3 and GAPDH expression. b Flow cytometry analysis of Med8A-R and IL6Rα^−/− cells for cell surface expression of IL6R, with corresponding IgG controls. Cell viability assay to assess the sensitivity of (c) STAT3^−/− and Med8A-R and (d) IL6R^−/− and Med8A-R cells to vincristine. As plotted is the mean ± SD of an experiment performed in triplicates, representative of at least 3 independent experiments. ***p < 0.001, two-way ANOVA with Bonferroni’s multiple comparison test. e Lysates of Med8A-R and IL6Rα^−/− untreated or treated with IL-6 were assessed for pY705-STAT3 and total STAT3 by western blot analysis. f Flow cytometry analysis of IL6R expression of Med8A-S, Med8A-R, STAT3^−/−, and IL6R^−/− cells. As plotted is the mean ± SD of the geometric mean fluorescence intensity (GMFI) for an experiment performed in triplicates; ***p < 0.001, one-way ANOVA with Bonferroni’s multiple comparison test.