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. 2020 Oct 14;12(12):2508–2520. doi: 10.1093/gbe/evaa219

Fig. 4.

Fig. 4

Intragenic recombination event between supergroups in argS gene. Intragenic recombination event was detected by the GARD method in argS (P value = 0.0006), and the inferred breakpoint is at 561 bp position in this gene. (A, B) Nucleotide ML trees for the 5′ region (1–561 bp) and 3′ region (positions 562–1,707 bp), respectively. argS genes from wDi, wNo, (B-Wolbachia), and wFol (E-Wolbachia) cluster with A supergroup (A). The supergroup classifications follow the color code in previous figures. Bootstrap values above 50 are shown in the figure. (A) argS from starting site to 561 bp, B-Wolbachia wDi, wNo, and E-Wolbachia wFol cluster with A supergroup with 19 bootstrap support, whereas (B) argS from 562 bp to stop site, wDi (B-Wolbachia) clusters with A supergroup with 79 bootstrap support, and wDacA (A-Wolbachia) clusters with E-Wolbachia with 35 bootstrap support, indicating intragenic recombination events; (C) nucleotides at selected positions (1–561 bp in argS) support the tree topology in (A); (D) nucleotides at selected positions (562–1,707 bp in argS) supported the tree topology in (B).