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. 2020 Dec 2;14:5349–5357. doi: 10.2147/DDDT.S256504

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© 2020 Wang et al.

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The effects of juglanin on the expression of transcription factors involved in adipogenesis in 3T3-L1 cells. Differentiated 3T3-L1 cells were treated with juglanin (0.5, 2.5, 5 μM) for 24 h. (A) The gene expression of PPAR-α, PPAR-γ, C/EBPα, C/EBPβ, and SREBP-1c was measured by real-time PCR; (B) The protein expression of PPAR-α, PPAR-γ, C/EBPα, C/EBPβ, and SREBP-1c was determined using Western blot analysis (**, ***, ****, P<0.01, 0.001, 0.0001 vs Vehicle control).