Figure 7.

Tetrandrine (Tet) inhibits osteoclastogenesis through the nuclear factor (NF)-κB signaling pathway. (a) Protein expression of the NF-κB signaling pathway. (b, c) Gray band levels of the phosphorylated inhibitor of NF-κB α (p-IκBα) (b) and phosphorylated p-65 (p-p65) (c) were analyzed using Image J software. (d) After 5 days of treatment with various Tet concentrations, the cells were lysed for Western blotting, and the gray band levels of p-p65 and IκBα were analyzed using ImageJ software (^∗p < 0.05, ^∗∗p < 0.01 compared with the vehicle group; n = 3 per group). Data are the means of at least three independent experiments with similar results.