Figure 3. Capping produced stably shortened nanofibers.

A) Images of Coil29 nanofibers in PBS assembled with different molar ratios of Coil29Caps, with Coil29 concentration kept constant at 2mM and annealed at 37°C for 1 hour prior to imaging. B) Box and whisker plots showing 25^th percentile, 50^th, and 75^th percentile with error bars at 10 and 90 percentile range and C) Length distributions of nanofibers formed with varying ratios of Coil29Caps (n=3 images per condition). Nanofiber lengths were compared using the Kruskal-Wallis test with Dunn’s multiple comparisons (***p<0.001, ****p<0.0001). D) Turbidity of 2mM solutions of nanofibers 3 hours after the addition of PBS, and immediately after extrusion. (n=3 independent samples, compared by one-way ANOVA with Tukey’s multiple comparisons.) E) Length distributions and F) polydispersity index of Coil29 nanofibers formed with and without Coil29Caps after annealing for up to three days, calculated from AFM measurements. (non-zero slope was non-significant for Coil29 fibers without Coil29Caps, **p<0.01 for Capped Coil29 fibers)