Skip to main content
. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Adv Mater. 2020 Aug 20;32(39):e2003310. doi: 10.1002/adma.202003310

Figure 4. Nanofiber capping improved cross-presentation in vitro and CD8+ T cell responses in vivo.

Figure 4.

A) Uptake of TAMRA-labeled Coil29 fibers (0.2 mM) by DC2.4 cells in vitro. (n=3 wells, compared by one-way ANOVA with Sidak’s multiple comparisons, **p<0.01). B) Mean Fluorescence Intensity of TAMRA+ cells from A (n=3 wells, compared by one-way ANOVA). C) Presentation of SIINFEKL peptide by mature BMDCs treated with Coil29 nanofibers bearing SIINFEKL peptides, measured by reporter B3Z CD8+ T cells. (n=3, compared by repeated measures ANOVA with Sidak’s multiple comparisons, *p<0.05, ***p<0.001) D) Endpoint IgG titer for mice immunized with Coil29 nanofibers bearing the OTII epitope OVA. Immunizations were given subcutaneously at time points indicated by arrows (n=5 mice, compared by repeated measures ANOVA). E) Numbers of CD8+ T cells specific to SIINFEKL epitope after 2 immunizations with SIINFEKL-Coil29 nanofibers. (n=10, pooled from 2 independent experiments, *p<0.05 compared by unpaired t-test with Welch’s correction).