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. 2020 Nov 23;11:569412. doi: 10.3389/fmicb.2020.569412

FIGURE 6.

FIGURE 6

The putative targets of GLV miRNA1. (A) Luciferase assays of the predicted GLV miRNA1 targets; Error bars represent 99% confidence intervals. The difference was significant [paired-samples t-test: p = 0.0002 (antisense GLV miRNA1), p = 0.0011 (5′-UTR), p = 0.010 (5′-gag), p = 0.002 (5′-UTR); N = 12]. (B) Real-time PCR analysis of the GLV miRNA1 cleavage efficiency on the putative targets, real-time PCR results at 6 and 12 h posttransfection. RFP is the reference gene, and 5′-UTR, 5′-gag, and 3′-UTR represent the putative GLV miRNA1 targets. Error bars represent 99% confidence intervals. The difference was significant [unpaired-samples t-test: p = 0.377 (5′-UTR); p < 0.001 (5′-gag); p < 0.001 (5′-UTR); N = 12]. **p < 0.01, ***p < 0.001.