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. 2020 Nov 23;11:574959. doi: 10.3389/fpls.2020.574959

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Copyright © 2020 Modrzejewski, Hartung, Lehnert, Sprink, Kohl, Keilwagen and Wilhelm.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rate of identified off-target effects assessing potential off-target sequences with (A) one mismatch (B) two mismatches (C) one and two mismatches to the target sequence considering the GC-content of the protospacer.