Table 2.

Study validity assessment criteria.

Question/criterion	Yes	Partly	No	Unclear
Study design/methods used
Did the CRISPR/Cas technique induce any on-target mutation?	Any on-target mutation was induced	–	No on-target mutation was induced	Lacking sufficient information to judge
Is a reference genome of the edited plant available?	Reference genome is available	–	Reference genome has not been fully sequenced/Sequencing error when analyzing potential off-target sequences	Lacking sufficient information to judge
Potential off-target sequences
Did a PAM follow the potential off-target sequences?*	All potential off-target sequences were followed by a PAM	Some potential off-target sequences were followed by a PAM	No potential off-target sequences were followed by a PAM	Lacking sufficient information to judge
Has the potential off-target sequence at least one mismatch to the on-target site	All potential off-target sequences have at least one mismatch	Some potential off-target sequences have at least one mismatch	All potential off-target sequences have no mismatch	Lacking sufficient information to judge
Are information about the potential off-target sequences provided? (number of mismatches, the position of mismatches to the PAM)	All information are provided	Information are partly provided	No information are provided	–