Any of these criteria answered with “No” or “Unclear” |
Did the CRISPR/Cas technique induce any on-target mutation? |
Did a PAM follow the potential off-target sequences? |
Has the potential off-target sequence at least one mismatch to the on-target site? |
Any of these criteria answered with “No” |
Is a reference genome of the edited plant available? |
Did sequencing errors occur? |
Are information about the potential off-target sequences provided? (number of mismatches, the position of mismatches to the PAM) |
Studies that were not assigned
low validity
were considered to have
medium validity
if any of the following factors applied
|
Any of the criteria answered with “Partly” |
Did a PAM follow the potential off-target sequences? |
Has the potential off-target sequence at least one mismatch to the on-target site |
Are information about the potential off-target sequences provided? (number of mismatches, the position of mismatches to the PAM) |
Potential off-target sequences within
medium validity
studies were handled as
medium/low validity
if any of the following factors was not applied
|
Any of the criteria answered with “No” |
Did a PAM follow the potential off-target sequences? |
Has the potential off-target sequence at least one mismatch to the on-target site |
Are information about the potential off-target sequences provided? (number of mismatches, the position of mismatches to the PAM) |
Potential off-target sequences within
medium validity
studies were handled as
medium/high validity
if all the following factors applied
|
All of the criteria answered with “Yes” |
Did a PAM follow the potential off-target sequences? |
Has the potential off-target sequence at least one mismatch to the on-target site |
Are information about the potential off-target sequences provided? (number of mismatches, the position of mismatches to the PAM) |