Table 4.
Description of independent Variables.
| # | Variable | Variable description | Level | Number of analyzed potential off-target sequences | Total (%) |
|---|---|---|---|---|---|
| x1 | Number of mismatches | One mismatch | 1 | 154 | 11 |
| Two mismatches | 2 | 218 | 16 | ||
| Three mismatches | 3 | 352 | 25 | ||
| Four or more mismatches | ≥4 | 671 | 48 | ||
| x2 | Position mismatch/es proximal to the PAM | (Any) mismatch within the seed sequence (Position 1-8 proximal to the PAM) | Yes | 862 | 62 |
| Any mismatch/es within the seed sequence | No | 533 | 38 | ||
| x3 | GC-content | <50% | Low | 505 | 36 |
| ≥50% | High | 890 | 64 | ||
| x4 | Delivery method | DNA-stable | DNA-stable | 1,229 | 88 |
| Others (DNA-transient, RNP, RNA) | Others | 166 | 12 | ||
| x5 | Nuclease variant | CRISPR/SpCas9 | SpCas9 | 1,218 | 87 |
| Further Nucleases | Others | 177 | 13 |