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. 2020 Nov 23;11:574959. doi: 10.3389/fpls.2020.574959

Table 5.

Overview on the validity assessment of all studies and the reason for validation.

Validity assessment Number of studies Reason for validity assessment outcomes Number of analyzed potential off-target sites
High validity 326 All study validity assessment criteria were rated as “Yes” High validity: 2,267
Medium validity 44 Some potential off-target sequences were followed by a PAM (n = 28)
Information are considerably incomplete (n = 15)
Only some potential off-target sequences show at least one mismatch (n = 7)
Medium/High validity: 4,149
Medium/Low validity: 154
Low validity 72 No on-target mutation was induced (n = 27)
Reference genome has not been fully sequenced/sequencing error when analyzing potential off-target sequences (n = 7)
Potential off-target sequences were not followed by a PAM (n = 15)
All potential off-target sequences have no mismatch (n = 5)
No information is provided (n = 23)
Overexpression of the sgRNA/Cas9 complex (n = 1)
Low validity: 197
Unclear validity 26 Lacking sufficient information to judge (n = 26) Unclear validity: 170