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. 2020 Nov 23;11:574959. doi: 10.3389/fpls.2020.574959

Table 6.

Overview about the characteristic items and frequency of studies rated as high and medium/high validity.

Study validity assessment n= Nuclease variant n= Delivery method n= Analyzed potential off-target sequences
High validity and 370 SpCas9 311 DNA-stable 283 5,999
medium/high validity
DNA-transient 18 78
RNPs 8 133
RNA 2 9
Cpf1 20 DNA-stable 20 62
eSpCas9 (1.1) 8 DNA-stable 4 26
DNA-transient 4 7
eSpCas9 (1.0) 8 DNA-stable 3 24
DNA-transient 5 8
SaCas9 6 DNA-stable 2 5
DNA-transient 4 4
Others 17 DNA-stable 9 47
DNA-transient 8 14

RNP, Ribonucleoproteins; RNA, Ribonucleic acid.