Table 6.
Overview about the characteristic items and frequency of studies rated as high and medium/high validity.
| Study validity assessment | n= | Nuclease variant | n= | Delivery method | n= | Analyzed potential off-target sequences |
|---|---|---|---|---|---|---|
| High validity and | 370 | SpCas9 | 311 | DNA-stable | 283 | 5,999 |
| medium/high validity | ||||||
| DNA-transient | 18 | 78 | ||||
| RNPs | 8 | 133 | ||||
| RNA | 2 | 9 | ||||
| Cpf1 | 20 | DNA-stable | 20 | 62 | ||
| eSpCas9 (1.1) | 8 | DNA-stable | 4 | 26 | ||
| DNA-transient | 4 | 7 | ||||
| eSpCas9 (1.0) | 8 | DNA-stable | 3 | 24 | ||
| DNA-transient | 5 | 8 | ||||
| SaCas9 | 6 | DNA-stable | 2 | 5 | ||
| DNA-transient | 4 | 4 | ||||
| Others | 17 | DNA-stable | 9 | 47 | ||
| DNA-transient | 8 | 14 |
RNP, Ribonucleoproteins; RNA, Ribonucleic acid.