Table 9.
Overview about the characteristics of analyzed potential off-target sequences using delivery methods to deliver the CRISPR system into the plant cells.
| Nuclease variant | Number of studies | Analyzed potential off-target sequences | Number of mismatches | Analyzed potential off-target sequences | Off-target effects identified |
|---|---|---|---|---|---|
| DNA-stable | 321 | 6,163 | 1 mismatch | 101 | 52 |
| 2 mismatches | 207 | 55 | |||
| 3 mismatches | 323 | 9 | |||
| ≥4 mismatches | 5,532 | 4 | |||
| DNA-transient | 39 | 111 | 1 mismatch | 51 | 39 |
| 2 mismatches | 7 | 1 | |||
| 3 mismatches | 18 | 2 | |||
| ≥4 mismatches | 35 | 1 | |||
| RNP | 8 | 133 | 1 mismatch | 1 | 1 |
| 2 mismatches | 4 | 1 | |||
| 3 mismatches | 13 | 0 | |||
| ≥4 mismatches | 115 | 0 | |||
| RNA | 2 | 9 | 1 mismatch | 1 | 1 |
| 2 mismatches | 0 | – | |||
| 3 mismatches | 2 | 0 | |||
| ≥4 mismatches | 6 | 0 |
RNP, Ribonucleoproteins; DNA, deoxyribonucleic acid; RNA, ribonucleic acid.