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. 2020 Nov 23;11:577927. doi: 10.3389/fneur.2020.577927

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Copyright © 2020 Wang, Sun, Ru, Wang, Huang, Ruan, Lin, Jin, Zhuge and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GSDMD is necessary for I/R induced pyroptosis and proinflammatory cytokine secretion in microglia BV2 cells. (A–D) Western blot analysis of the relative expression of cleaved-GSDMD (B), active caspase-1 (p20) (C), and caspase-11 (D) in microglia after OGD/R. (E,F) Relative expression of cleaved-GSDMD post shRNA-GSDMD interference or GSDMD overexpression in microglia. n = 5. (G,H) Relative expression of caspase-1 and caspase-11 in microglia post GSDMD silence or GSDMD overexpression. n = 5. (I,J) The expression of mature IL-1β and IL-18 in microglia BV2 cells were measured by ELISA at 4 h post OGD/R. n = 3. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the Sham group or relative group. GSDMD, gasdermin D; I/R, ischemia reperfusion; OGD, oxygen glucose deprivation.