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. 2020 Nov 23;8:586296. doi: 10.3389/fcell.2020.586296

FIGURE 1.

FIGURE 1

Morphometric-phenotype assessment of larvae at 3 days post fertilization (dpf) and 5 dpf. (A) Larval fish were imaged in three orientations (dorsal, right lateral, and left lateral) using the VAST system. Morphometric features were obtained manually using Fiji [body length (L), eye area (EA), telencephalon (Tel), and optic tectum (OT)] and automatically using FishInspector (L, EA, head width, pericardium area, and yolk area). (B) Manual and automated measurements were compared with L and EA (averaged between both eyes) showing high correlations at 3 dpf (n = 97) and 5 dpf (n = 73) (Pearson correlation). Blue line represents the fit of a linear model with 95% confidence intervals. (C) Boxplots pictured represent distribution of measurements for larval morphometric features obtained via automated (L and EA) and manual (Tel and OT) methods. Boxplots include the median value (dark line), and the whiskers represent a 1.5 interquartile range.