FIGURE 5.

slc7a5 CRISPR hom mutants exhibit moderate developmental delay and enhanced seizures with flashing lights. (A) The zebrafish mutant line slc7a5^tup1/tup1 was generated using a single gRNA targeting the first exon of the gene resulting in an 18-bp deletion leading to a frameshift. (B) PCA of combined morphometric traits at 3 and 5 dpf colored by genotype. (C) The three traits found to be significantly increased in het (purple) and hom (red) mutant versus WT (gray) siblings included L, EA, and OT at 3 dpf, displayed as box plots with total numbers of larvae measured indicated in the EA plot (^∗p < 0.05; ^∗∗p < 0.01 using a Tukey post hoc test). Boxplots include the median value (dark line), and the whiskers represent a 1.5 interquartile range. (D) Behavioral data from DanioVision motion tracking shows significant increased distance moved over the final 15 min following flashing lights for hom versus WT siblings (^∗p < 0.05 using a Dunn’s multiple comparison post hoc test). The left plot indicates the mean distance per minute with error bars represented as standard error of the mean. Indicated by the arrow, at 45 min fish were subjected to light flashing (see Materials and Methods). The box plot shows the mean for the final 15 min of the behavioral assay, and the median value (dark line) and the whiskers represent a 1.5 interquartile range.