TABLE 1.

Summary effect size and significance for each treatment per measurement.

Morphometric measurement (5 dpf)	Complete model*			VAST at 3 dpf		Behavior		PTZ
	F (df)	p-value	R2	Beta	p-value	Beta	p-value	Beta	p-value
Average EA (mm2)	43.98 (9, 617)	p < 2.2 × 10–16	0.382	−0.00553	4.85 × 10–4	0.0006	0.477	−0.0020	8.77 × 10–3
				Yes = 228, No = 399		Yes = 566, No = 61		10 mM = 337, 0 mM = 290
L (mm)	69.98 (9, 617)	p < 2.2 × 10–16	0.498	−0.06075	0.0525	0.0316	0.068	−0.0923	3.89 × 10–9
				Yes = 228, No = 399		Yes = 566, No = 61		10 mM = 337, 0 mM = 290
Tel (mm)	35.71 (9, 617)	p < 2.2 × 10–16	0.333	−0.01152	0.0136	−0.005386	0.037	−0.0080	5.53 × 10–4
				Yes = 228, No = 399		Yes = 566, No = 61		10 mM = 337, 0 mM = 290
OT (mm)	39.84 (9, 615)	p < 2.2 × 10–16	0.359	−0.01583	7.77 × 10–4	−0.0004	0.866	−0.0071	2.28 × 10–3
				Yes = 226, No = 399		Yes = 564, No = 61		10 mM = 335, 0 mM = 290
1 h average distance (mm)	186.5 (8, 666)	p < 2.2 × 10–16	0.688	9.916	0.046	–	–	119.6	<2 × 10–16
				Yes = 219, No = 456		–		10 mM = 377, 0 mM = 298
15 min average distance (mm)	184.3 (8, 666)	p < 2.2 × 10–16	0.685	11.712	0.017	–	–	122.7	<2 × 10–16
				Yes = 219, No = 456		–		10 mM = 377, 0 mM = 298

^∗The “complete model” also included “Experiment” as a variable to reduce inter-batch effects (see section “Materials and Methods”). Values in bold represent p < 0.05.