Table 4.
Relative expression levels of tissue plasminogen activator (t-PA) mRNA, plasminogen activator inhibitor type-1 (PAI-1) mRNA, and ICAM-1 mRNA in human umbilical vein endothelial cells (HUVECs) stimulated by TNF-α in different treatment groups (mean ± SD)a.
| Shear stress (Pa) | CPSM (μg·ml−1) | t-PA mRNA | PAI-1 mRNA | ICAM-1 mRNA | |||
|---|---|---|---|---|---|---|---|
| Control group | 2.60 ± 0.23 | 0.62 ± 0.20 | 0.69 ± 0.13 | ||||
| Model group | 1.03 ± 0.16 | 1.84 ± 0.24 | 1.81 ± 0.36 | ||||
| 0.1 | 0 | 0.83 ± 0.22 | 1.76 ± 0.55 | 1.81 ± 0.31 | |||
| 0.1 | 150 | 1.01 ± 0.20 | 1.15 ± 0.21 | 1.59 ± 0.31 | |||
| 0.1 | 300 | 1.34 ± 0.24 | 0.67 ± 0.10 | 0.89 ± 0.21 | |||
| 0.9 | 0 | 0.93 ± 0.16 | 1.55 ± 0.13 | 2.14 ± 0.12 | |||
| 0.9 | 150 | 1.13 ± 0.22 | 0.93 ± 0.20 | 0.99 ± 0.12 | |||
| 0.9 | 300 | 2.34 ± 0.58 | 0.71 ± 0.15 | 1.11 ± 0.26 | |||
| Two-way ANOVA | F | p | F | p | F | p | |
| Shear stress | 5.72 | 0.03 | 0.95 | 0.35 | 0.01 | 0.92 | |
| CPSM | 11.57 | 0.01 | 19.16 | < 0.001 | 26.82 | < 0.001 | |
| CPSM × shear stress | 3.01 | 0.08 | 0.45 | 0.65 | 6.858 | 0.01 | |
Relative expression levels of t-PA mRNA, PAI-1mRNA, and ICAM-1 mRNA in HUVECs were calculated using 2−ΔΔCt method from triplicate preformation.
a
All data are expressed as the mean ± SD. The GLM procedure was applied for a two-way ANOVA to test for synergy. Comparisons between each pair of factor levels were analyzed using a Bonferroni post hoc test. A value of p < 0.05 was considered statistically significant.