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. 2020 Nov 23;11:603081. doi: 10.3389/fphys.2020.603081

FIGURE 8.

FIGURE 8

Search and identification of the downstream target genes of miR-769-5p. (A) Protein expression of TGFBR1 in HSFs after transfection of miR-769-5p mimic from 25 to 100 nM. (B) MRNA expression of TGFBR1 in HSFs after transfection of miR-769-5p mimic from 25 to 100 nM. *P < 0.05 comparing with NC group. **P < 0.01 comparing with NC group. ***P < 0.001 comparing with NC group. (C) Target site of miR-769-5p in TGFBR1 mRNA 3′-UTR was predicted and luciferase report vector of relative wild and mutant type were built. (D) Fluorescence activity of wild type and mutant type after transfection of miR-769-5p mimic. GLO, pmirGLO vector; WT, wild type; MUT, mutant type. *P < 0.05. NS, no statistical difference.