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. 2020 Dec 6;11(12):1037. doi: 10.1038/s41419-020-03255-6

Fig. 3. SphK1 inhibition suppresses p300 expression and HDAC4 translocation.

Fig. 3

RAW264.7 cells were pretreated with SKI-5C (10 μM for 24 h) and then stimulated with LPS (1 μg/ml for 16 h). A Colorimetric assay of nuclear HATs activity. B Colorimetric assay of HDACs activity. C Ratios of nuclear HATs to HDACs activity. D Immunoblot analysis of the expression of nuclear CBP, PCAF, p300, HDAC1, and HDAC4. E–I Quantitative analysis of the expression of nuclear CBP (E), PCAF (F), p300 (G), HDAC1 (H), and HDAC4 (I). Data represent three independent experiments. J Immunoblot analysis of cytoplasmic HDAC1 expression. Data represent three independent experiments. K Immunoblot analysis of cytoplasm HDAC4 expression. The data represent three independent experiments. L Kupffer cells were pretreated with SKI-5C (10 μM for 24 h) and then stimulated with LPS (1 μg/ml for 16 h). HDAC4 intracellular translocation in Kupffer cells was analyzed by immunofluorescence assay. Data were expressed as the mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001. HDAC4, nuclear histone deacetyltransferase 4; p300, E1A-associated protein p300; HATs, nuclear histone acetyltransferases; HDACs, nuclear histone deacetyltransferases; CBP, CREB-binding protein; PCAF, p300/CBP-associated factor; and HDAC1, nuclear histone deacetyltransferase 1.