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. 2020 Dec 6;11(12):1037. doi: 10.1038/s41419-020-03255-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — RAW264.7 cells were pretreated with SKI-5C (10 μM for 24 h) and then stimulated with LPS (1 μg/ml for 16 h). A Immunoblot analysis of Phospho-HDAC4. Data represent three independent experiments. B Colorimetric assay of CaMKII activity. C Immunoblot analysis of CaMKII-δ expression. Data represent three independent experiments. D Immunoblot analysis of phospho-CaMKII-δ. Data represent three independent experiments. E Co-immunoprecipitation analysis of the interaction between SphK1 and CaMKII-δ in LPS-stimulated RAW264.7 cells. F Pull-down analysis of HA, HA-SphK1, and His-CaMKII-δ proteins. Data are expressed as the mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001. CaMKII-δ, calcium/calmodulin protein kinase II-δ.