TABLE 1.
Descriptive statistics of the 15 electrophysiological parameters in neurons recorded at the edge of cortical organoids.
| Age, treatment | Cm (pF) | Rm (GΩ ) | Vrest (mV) | AP frequency (Hz) | AP amplitude (mV) | AP ½ width (ms) | AP threshold (mV) | AP rise slope (mV/ms) | N | |||||||||
| Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | |||
| 12-wk, control | 2.89 | 0.91 | 8.15 | 3.77 | –55.3 | 3.1 | 1.73 | 1.16 | 48.7 | 5.5 | 7.59 | 3.30 | –27.6 | 2.7 | 31.8 | 9.2 | 21–23 | |
| 16-wk, control | 5.62 | 1.50 | 6.40 | 2.53 | –55.3 | 4.7 | 4.05 | 1.98 | 60.8 | 7.5 | 3.17 | 0.82 | –25.9 | 3.3 | 56.1 | 12.5 | 15–20 | |
| 16-wk, 1 μM meth | 5.73 | 0.94 | 6.41 | 2.13 | –53.6 | 3.0 | 4.25 | 1.67 | 60.6 | 6.5 | 3.26 | 0.66 | –25.2 | 1.7 | 56.0 | 9.9 | 8–10 | |
| 16-wk, 10 μM meth | 3.82 | 1.04 | 9.19 | 3.70 | –49.8 | 4.8 | 1.79 | 0.98 | 50.0 | 6.2 | 4.52 | 1.58 | –22.9 | 2.4 | 43.0 | 9.5 | 19–29 | |
| 20-wk, control | 7.85 | 1.42 | 3.23 | 1.52 | –61.7 | 3.2 | 13.00 | 2.48 | 62.9 | 6.1 | 2.62 | 0.75 | –27.7 | 3.8 | 63.5 | 12.4 | 9–12 | |
| 20-wk, 1 μM meth | 6.7 | 1.37 | 5.33 | 2.04 | –55.4 | 2.3 | 6.85 | 3.7 | 59.6 | 8.4 | 3.03 | 1.30 | –24.9 | 2.4 | 60.8 | 9.7 | 11–14 | |
| 20-wk, 10 μM meth | 5.24 | 0.96 | 7.89 | 2.45 | –55.3 | 5.1 | 2.54 | 1.13 | 51.1 | 5.3 | 3.52 | 0.78 | –24.3 | 3.3 | 47.1 | 10.4 | 13–14 | |
| 24-wk, control | 8.39 | 1.65 | 2.98 | 0.99 | –60.1 | 4.0 | 11.72 | 4.08 | 68.1 | 8.0 | 2.56 | 0.90 | –33.1 | 2.6 | 74.7 | 20.6 | 14–15 | |
| 24-wk, 1 μM meth | 6.49 | 1.28 | 5.70 | 1.68 | –55.4 | 3.8 | 6.55 | 3.53 | 66.2 | 7.2 | 3.20 | 1.13 | –28.9 | 2.9 | 66.9 | 13.7 | 11–12 | |
| 24-wk, 10 μM meth | 5.51 | 0.84 | 7.25 | 2.59 | –49.6 | 3.9 | 4.73 | 2.41 | 62.2 | 10.3 | 3.29 | 1.07 | –30.7 | 2.5 | 54.2 | 12.5 | 11–13 | |
| Age, treatment | AP decay slope (mV/ms) | AHP amplitude (mV) | peak INa (pA) | V0:5-activ (mV) | V0:5-inactiv (mV) | peak IKD (pA) | peak IKA (pA) | N | ||||||||||
| Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | |||||
| 12-wk, control | –16.1 | 8.6 | 5.2 | 4.5 | –394.7 | 233.1 | –34.0 | 4.3 | –50.0 | 3.7 | 201.5 | 140.6 | 528.2 | 253.7 | 21–23 | |||
| 16-wk, control | –41.7 | 7.5 | 9.8 | 2.6 | –846.9 | 329.6 | –37.3 | 2.8 | –38.7 | 3.7 | 966.1 | 211.6 | 644.2 | 160.7 | 15–20 | |||
| 16-wk, 1 μM meth | –44.4 | 8.8 | 10.9 | 3.4 | –815.3 | 468.3 | –35.1 | 1.7 | –40.5 | 2.4 | 704.1 | 768.7 | 466.7 | 557.2 | 8–10 | |||
| 16-wk, 10 μM meth | –32.1 | 6.9 | 4.7 | 3.3 | –463.7 | 236.3 | –31.3 | 3.5 | –40.8 | 4.3 | 594.2 | 251.1 | 526.1 | 210.9 | 19–29 | |||
| 20-wk, control | –48.8 | 9.0 | 14.3 | 4.3 | −1, 513.0 | 593.3 | –38.6 | 5.4 | –36.6 | 4.8 | 1, 738.1 | 834.8 | 982.1 | 454.5 | 9–12 | |||
| 20-wk, 1 μM meth | –49.5 | 8.6 | 13.8 | 4.2 | −1, 095.3 | 377.4 | –34.4 | 4.9 | –37.7 | 6.0 | 1, 162.7 | 485.9 | 1, 012.5 | 495.6 | 11–14 | |||
| 20-wk, 10 μM meth | –36.1 | 6.1 | 7.8 | 3.5 | –615.1 | 270.1 | –33.8 | 5.7 | –42.4 | 3.1 | 651.4 | 183.2 | 661.9 | 391.3 | 13–14 | |||
| 24-wk, control | –54.7 | 15.1 | 16.4 | 4.1 | −1, 766.9 | 476.7 | –45.6 | 4.9 | –38.9 | 3.4 | 2, 164.8 | 733.2 | 930.2 | 263.3 | 14–15 | |||
| 24-wk, 1 μM meth | –46.1 | 8.1 | 12.4 | 3.8 | −1, 061.4 | 423.8 | –40.0 | 3.8 | –41.6 | 4.1 | 1, 208.2 | 494.3 | 774.5 | 283.6 | 11–12 | |||
| 24-wk, 10 μM meth | –38.9 | 5.3 | 12.6 | 3.9 | –850.3 | 241.5 | –39.0 | 5.3 | –42.5 | 5.7 | 922.3 | 361.2 | 677.6 | 337.2 | 11–13 | |||
Cm, membrane capacitance; Rin, input resistance, Vrest, resting membrane potential; AP, action potential; AHP, afterhyperpolarization; V0.5–activ, half-maximal activation voltage of Na+ channels; V0.5–inactiv, half-maximal inactivation voltage of Na+ channels.