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. 2020 Nov 23;8:566504. doi: 10.3389/fcell.2020.566504

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Copyright © 2020 He, Zheng, Luo, Hong, Su and Cai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of semicircular canal formation in PHF8 morphant. (A–E) The overall morphology of semicircular canal in control embryos (ConMO; A), PHF8 morphants (PHF8-MO; B–D), and PHF8-MO co-injected with PHF8-mRNA embryos (PHF8-MO + PHF8-mRNA; E) at 2 dpf (A1–E1), 3 dpf (A2–E2), 4 dpf (A3–E3), and 5 dpf (A4–E4). Black arrowheads indicate the junction of the anterior protrusion (ap) and the anterior bulge (ab) and the junction of the posterior bulge (pb) and posterior protrusion (pp). Asterisks point out the junction between the ventral bulge (vb) and ventral protrusion (vp). Red arrowheads mark disrupted projections. The locations of the anterior otolith (ao) and posterior otolith (po) are implied. All pictures are lateral views with the anterior to the left and the dorsal side up. Scale bar, 50 μm.