Figure 1.

Weight change, survival rate, and bacterial burden of klotho wildtype (WT) and knockout (KO) mice infected with Acinetobacter baumannii. A. baumannii ATCC 19606 (10⁸ CFU/mouse) was inoculated intravenously into 6-week-old klotho WT and KO mice. (A) Summarized results show weight changes of uninfected klotho WT and KO mice, and represent the mean ± SEM (WT, n = 5; KO, n = 5). (B) Summarized results show weight changes of klotho WT and KO mice after infection with A. baumannii, and represent the mean ± SEM (WT, n = 14; KO, n = 15). These data are compiled from three independent experiments. Asterisks indicate statistically significant differences (^** P < 0.01; ^* P < 0.05, klotho WT mice vs. klotho KO mice; Student’s t-test). (C) Survival curves of klotho WT and KO mice after infection with A. baumannii are shown (WT, n = 14; KO, n = 15). These data are compiled from three independent experiments. Comparison of survival curves with log rank test yielded statistical significance (^* P = 0.04). Summarized results show (D) bacterial count in the lungs, (E) spleen, and (F) blood of klotho WT (n = 12) and KO (n = 12) mice after infection with A. baumannii. These data are compiled from two independent experiments. There are no significant differences between klotho WT and KO mice. Each symbol represents one mouse. The mean value is shown as a horizontal solid line.