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. 2020 Nov 23;11:601614. doi: 10.3389/fimmu.2020.601614

Figure 7.

Figure 7

Number of innate immune cells in the blood and proinflammatory cytokine expression levels in the sera of klotho wildtype (WT) and knockout (KO) mice before and after infection with Acinetobacter baumannii. Summarized results show the number of (A) neutrophils, (B) eosinophils, (C) monocytes, and (D) macrophages in the blood of A. baumannii-infected klotho WT and KO mice. Line graphs were compiled from four independent experiments (n = 16 for WT and n = 16 for KO mice), and represent the mean ± SEM. Asterisks indicate statistically significant differences (* P < 0.05, klotho WT mice before infection vs. klotho WT mice after infection, Student’s t-test; †† P < 0.01, klotho KO mice before infection vs. klotho KO mice after infection, Student’s t-test; ‡‡ P < 0.01, klotho WT mice vs. klotho KO mice, Student’s t-test). The production of (E) TNF-α, (F) IL-6, (G) IL-10, (H) IFN-γ, (I) CXCL1, (J) CCL2, and (K) CCL7 in the sera of klotho WT and KO mice before and after infection with A. baumannii. Bar graph data represent the mean ± SEM and were compiled from four independent experiments (n = 16 for WT and n = 16 for KO mice). Asterisks indicate statistically significant differences (**P < 0.01; *P < 0.05, before infection vs. after infection, Student’s t-test; †† P < 0.01; † P < 0.05, klotho WT mice vs. klotho KO mice, Student’s t-test).