Table 3. Comparison of thickness of different retinal layers (INL and IPL/ONL) on day 56 after high intraocular pressure induced retinal ischemia reperfusion damage (IR injury) in eight experimental groups.
| Retinal thickness (μM) | −6 | −5 | −4 | −3 | −2 | −1 | ONH | 1 | 2 | 3 | 4 | 5 | 6 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| INL | Normal control (n=6) | 21.52 ± 0.62 a) | 23.91 ± 0.48 a) | 27.71 ± 7.38 a) | 31.09 ± 9.38 a) | 32.47 ± 5.37 a) | 32.21 ± 5.74 a) | 0 | 28.99 ± 9.22 a) | 40.73 ± 3.31 a) | 42.61 ± 1.70 a) | 36.89 ± 7.79 a) | 34.26 ± 2.24 a) | 28.56 ± 2.60 a) |
| IR injury (n=6) | 16.63 ± 0.19 a) | 17.69 ± 0.33 a) | 15.88 ± 3.67 b) | 16.80 ± 0.56 b) | 18.75 ± 0.11 b) | 24.10 ± 0.35 a) | 0 | 13.26 ± 1.75 a) | 10.46 ± 1.22 b) | 18.61 ± 3.86 b) | 19.81 ± 0.86 b) | 16.74 ± 7.23 a) | 18.54 ± 3.06 a) | |
| Blended (500 mg/kg, n=6) | 26.12 ± 8.24 a) | 36.45 ± 5.42 b) | 36.12 ± 6.5 a) | 40.56 ± 8.10 a) | 34.50 ± 4.50 a) | 32.10 ± 6.41 a) | 0 | 31.54 ± 5.10 a) | 35.16 ± 6.40 a) | 42.13 ± 4.12 a) | 34.55 ± 3.75 a) | 30.45 ± 5.40 a) | 27.23 ± 7.45 a) | |
| Blended (250 mg/kg, n=6) | 28.64 ± 12.18 a) | 33.46 ± 13.58 b) | 37.37 ± 3.21 a) | 47.25 ± 7.29 a) | 36.34 ± 3.06 a) | 30.08 ± 4.53 a) | 0 | 32.94 ± 2.40 a) | 37.55 ± 7.17 a) | 40.89 ± 12.08 a) | 36.59 ± 1.93 a) | 29.95 ± 3.78 a) | 25.11 ± 8.60 a) | |
| Blended (100 mg/kg, n=6) | 25.41 ± 4.23 a) | 36.45 ± 5.73 b) | 37.98 ± 5.44 a) | 35.24 ± 4.12 a) | 34.60 ± 2.30 a) | 33.41 ± 7.40 a) | 0 | 36.78 ± 3.21 a) | 39.43 ± 6.78 a) | 40.12 ± 3.48 a) | 35.45 ± 5.52 a) | 34.60 ± 3.11 a) | 35.10 ± 5.40 a) | |
| Milled (500 mg/kg, n=6) | 33.12 ± 6.14 b) | 34.64 ± 5.30 b) | 34.56 ± 4.70 a) | 37.10 ± 3.45 a) | 36.41 ± 6.44 a) | 36.56 ± 4.17 a) | 0 | 40.20 ± 7.10 a) | 49.50 ± 5.14 a) | 36.40 ± 6.42a) | 37.90 ± 2.41 a) | 34.10 ± 3.80 a) | 33.24 ± 4.10 a) | |
| Milled (250 mg/kg, n=6) | 34.20 ± 3.33 b) | 32.82 ± 1.64 b) | 33.70 ± 3.25 a) | 36.97 ± 2.79 a) | 37.51 ± 5.08 a) | 40.57 ± 7.95 a) | 0 | 60.25 ± 13.32 b) | 56.16 ± 5.34 c) | 52.64 ± 9.24 c) | 54.14 ± 9.24 c) | 49.95 ± 7.82 b) | 51.85 ± 8.03 b) | |
| Milled (100 mg/kg, n=6) | 30.40 ± 6.10 b) | 33.54 ± 5.40 b) | 36.12 ± 3.40 a) | 37.56 ± 4.76 a) | 37.43 ± 6.30 a) | 38.23 ± 5.40 a) | 0 | 43.15 ± 10.30 a) | 47.50 ± 8.10 a) | 42.68 ± 6.40 a) | 42.12 ± 3.47 a) | 37.45 ± 4.22 a) | 32.12 ± 6.41 a) | |
| IPL/ONL | Normal control (n=6) | 0.93 ± 0.13 a) | 0.99 ± 0.01 a) | 1.12 ± 0.09 a) | 1.21 ± 0.02 a) | 1.15 ± 0.10 a) | 1.10 ± 0.11 a) | 0 | 1.21 ± 0.35 a) | 1.20 ± 0.15 a) | 1.06 ± 0.10 a) | 1.13 ± 0.17 a) | 1.06 ± 0.28 a) | 1.12 ± 0.14 a) |
| IR injury (n=6) | 0.24 ± 0.03 b) | 0.28 ± 0.02 b) | 0.25 ± 0.01 b) | 0.24 ± 0.02 b) | 0.30 ± 0.00 b) | 0.27 ± 0.04 b) | 0 | 0.21 ± 0.01 b) | 0.26 ± 0.07 b) | 0.27 ± 0.02 b) | 0.30 ± 0.03 b) | 0.22 ± 0.00 b) | 0.23 ± 0.01 b) | |
| Blended (500 mg/kg, n=6) | 0.88 ± 0.21 a) | 0.86 ± 0.09 a) | 1.02 ± 0.12 a) | 0.97 ± 0.34 a) | 0.95 ± 0.17 a) | 0.97 ± 0.12 a) | 0 | 0.93 ± 0.17 a) | 0.94 ± 0.11 a) | 0.99 ± 0.08 a) | 1.04 ± 0.06 a) | 0.97 ± 0.22 a) | 0.93 ± 0.18 a) | |
| Blended (250 mg/kg, n=6) | 0.89 ± 0.09 a) | 1.05 ± 0.19 a) | 1.07 ± 0.32 a) | 1.08 ± 0.29 a) | 0.95 ± 0.22 a) | 0.96 ± 0.22 a) | 0 | 0.89 ± 0.15 a) | 0.97 ± 0.07 a) | 1.07 ± 0.13 a) | 1.14 ± 0.09 a) | 1.12 ± 0.12 a) | 1.06 ± 0.26 a) | |
| Blended (100 mg/kg, n=6) | 0.96 ± 0.13 a) | 1.01 ± 0.17 a) | 1.04 ± 0.07 a) | 0.97 ± 0.24 a) | 0.92 ± 0.17 a) | 0.86 ± 0.15 a) | 0 | 0.87 ± 0.23 a) | 0.94 ± 0.14 a) | 1.03 ± 0.30 a) | 1.12 ± 0.20 a) | 1.09 ± 0.14 a) | 1.03 ± 0.19 a) | |
| Milled (500 mg/kg, n=6) | 0.75 ± 0.16 a) | 0.73 ± 0.17 a) | 0.80 ± 0.20 a) | 0.71 ± 0.18 a) | 0.76 ± 0.23 a) | 0.83 ± 0.08 a) | 0 | 0.84 ± 0.04 a) | 0.82 ± 0.18 a) | 0.79 ± 0.13 a) | 0.75 ± 0.06 a) | 0.81 ± 0.12 a) | 0.84 ± 0.04 a) | |
| Milled (250 mg/kg, n=6) | 0.89 ± 0.18 a) | 0.82 ± 0.13 a) | 0.74 ± 0.18 a) | 0.77 ± 0.17 a) | 0.83 ± 0.29 a) | 0.79 ± 0.23 a) | 0 | 0.81 ± 0.14 a) | 0.84 ± 0.23 a) | 0.76 ± 0.25 a) | 0.90 ± 0.33 a) | 0.77 ± 0.20 a) | 0.95 ± 0.10 a) | |
| Milled (100 mg/kg, n=6) | 0.73 ± 0.08 a) | 0.76 ± 0.15 a) | 0.81 ± 0.07 a) | 0.76 ± 0.21 a) | 0.73 ± 0.19 a) | 0.82 ± 0.12 a) | 0 | 0.83 ± 0.14 a) | 0.87 ± 0.16 a) | 0.74 ± 0.04 a) | 0.73 ± 0.16 a) | 0.74 ± 0.15 a) | 0.78 ± 0.20 a) | |
Eight experimental groups include control group, IR injury group, blended groups: 500 mg/kg, 250 mg/kg, 100 mg/kg; and milled (submicron) groups: 500 mg/kg, 250 mg/kg, 100 mg/kg. IPL, inner plexiform layer; INL, inner nuclear layer; ONL, outer nuclear layer, IPL/ONL, the ratio of IPL/ONL. ONH, optic nerve head. Data are shown as Mean ± SD and evaluation by one-way ANOVA followed by Scheffe’s multiple comparison test. n=6 in each group. The data with different superscript alphabetical letters indicate significantly statistical difference (P<0.05) between groups. The data with the same superscript letters indicate no significant difference (P>0.05) between groups.