Table 2.
Analytical validation pooled sample information and statistical results of assay validations with gray whale fecal extracts in progesterone, testosterone, cortisol and T3 immunoassays
| Assay | Parallelism sample pool | Parallelism results | Accuracy sample pool | Accuracy results |
|---|---|---|---|---|
| Progesterone |
N = 3 (A, B, C) |
F
1,5 = 1.253 P = 0.405 |
N = 3 (A, B, C) |
Slope = 0.921 r2 = 0.997 |
| Testosterone |
N = 7 (A, B, C, D, E, F, G) |
F
1,4 = 1.070 P = 0.431 |
N = 4 (B, E, F, G) |
Slope = 0.966 r2 = 0.997 |
| Cortisol |
N = 5 (A, B, C, D, E) |
F
1,6 = 1.023 P = 0.500 |
N = 4 (A, B, C, F) |
Slope = 1.269 r2 = 0.992 |
| T3 |
N = 3 (E, F, G) |
F
1,6 = 1.386 P = 0.353 |
N = 3 (E, F, G) |
Slope = 1.026 r2 = 0.996 |
Legend: (A) Whale of undetermined maturity and sex with minimum age of 10 years in 2017 (whale ID: ErPNW-323), (B) resting female with minimum age of 21 years in 2017 (whale ID: ErPNW-19), (C) mature male with minimum age of 23 years in 2017 (whale ID: ErPNW-256), (D) mature whale of unknown sex with minimum age of 21 years in 2017 (whale ID: ErPNW-287), (E) immature male with minimum age of 1 year in 2016 (whale ID: ErPNW-223), (F) immature male of unknown age (ID: STRAND1), (G) immature male of unknown age (ID: STRAND2).