Fig. 5.

Crystal structures of DmNbr and AaNbr EXO domains. (A) Overall structure of DmNbr EXO (DmEXO). The structure is shown as cartoon and colored salmon. (B) Electrostatic surface potentials of DmEXO. Electrostatic surface potentials were calculated by APBS and contoured from −4 kT/e (red) to +4 kT/e (blue). The catalytic pocket is highlighted by yellow box. (C) Close-up view of the DEDD-Y motif in the catalytic pocket of DmEXO. The catalytic residues with D435A mutation are shown as sticks and colored magenta. (D) Overall structure of AaNbr EXO (AaEXO). The structure is shown as cartoon and colored cyan. (E) Electrostatic surface potentials of AaEXO. (F) Structural alignment of DmEXO and AaEXO. Structures are shown as ribbons and colored salmon and cyan, respectively.