Fig. 5.
Rox8 promotes yki mRNA decay via miR-8. (A) Overexpression of PremiR-8 in S2 cultured cells resulted in reduced Yki protein level. (B) Fluorescent images of third instar larval wing discs. Ectopic Rox8-mediated Yki reduction was dramatically inhibited in miR-8 null mutants. (C) Light micrographs of Drosophila adult eyes are shown. Rox8 overexpression suppressed the enlarged eye size caused by YkiUTR, which was cancelled by depletion of miR-8. Magnification for C: 8×. (D) miR-8–3p was specifically enriched in the Flag (Rox8)-IP reaction. (E) yki mRNA is a target of miR-8. (F) Overexpression of Rox8 enhanced the binding ability of miR-8 to yki mRNA. (G) Depletion of Rox8 decreased miR-8 binding to yki mRNA. (H) Proposed model of Rox8-mediated yki mRNA degradation by recruiting or stabilizing miR-8–loaded RISC into yki mRNA 3′ UTR. ***P < 0.001, *P < 0.05.