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. Author manuscript; available in PMC: 2020 Dec 7.
Published in final edited form as: Biochem Biophys Res Commun. 2016 Oct 13;480(2):187–193. doi: 10.1016/j.bbrc.2016.10.023

Fig. 2. LGP2 but not RIG-I or MDA5 co-precipitates with TRBP in HEK293T cells.

Fig. 2.

HA-tagged TRBP plasmid was co-introduced with FLAG-tagged LGP2, -RIG-I, MDA5 or Dicer plasmid in HEK293T cells and the lysate was subjected to immuno-precipitation with HA beads to co-purity FLAG-tagged proteins for Western blot (A). HA-tagged TRBP plasmid was co-introduced with FLAG-tagged LGP2 or MDA5 plasmid in HEK293T cells and cells were infected or mock infected with EMCV (MOI = 1) for 9.5 h. The lysate was subjected to immunoprecipitation as in A (B). The intensity was determined by densitometric analysis using Quantity One software (Bio-Rad). L929 cells were mock infected or infected with EMCV (MOI = 1) and the lysate was subjected to immunoprecipitation with anti-TRBP antibody or control rabbit IgG to co-purity endogenous LGP2 protein. The precipitant was probed with anti-LGP2 antibody for Western blot analysis (C).