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(A) Acute alcohol intoxication decreased the number of total Gr1+ cells in the bone marrow 24 hours following i.v. challenge with E. coli. Control: i.p. saline; alcohol: i.p. alcohol; saline: i.v. saline; E. coli: i.v. E. coli. Values are mean ± SEM. N = 6 in each group. Bars with different letters in the panel are statistically different (p < 0.05). (B) Gating strategy of flow cytometry for analyzing cell subsets along granulocyte lineage development.
