Fig. 6.

THP-1-derived M2 macrophages promote EMT in ICC cells. a Morphological changes during EMT of HuCCT1 and Huh28 cells after coculture with M2 macrophages. IF staining for EMT markers in HuCCT1 and Huh28 cells cocultured with THP-1-derived M2 macrophages. Green and red represent E-cadherin and vimentin marker staining, respectively, and the blue signal represents DAPI-stained nuclei. Bar = 1 mm. b qRT-PCR analyses of E-cadherin, N-cadherin, vimentin, Snail, Slug, Twist and ZEB1 expression in ICC cells cocultured with THP-1-derived M2 macrophages. c Western blotting analyses of E-cadherin and vimentin expression in ICC cells cocultured with M2 macrophages treated with IL-10 and its neutralizing antibody. The data are presented as the mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001. Bar = 1 mm