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. 2020 Dec 7;17:103. doi: 10.1186/s12986-020-00530-3

Fig. 4.

Fig. 4

Cell viability was detected by CCK-8 assay in folate-deficient mESCs pretreated with LY294002 (LY) or PD98059 (PD). DMSO was the control. ac Folate-deficient mESCs treated by LY294002 with different concentration shows various cell viability in dose-dependent and time-dependent manners, ***P < 0.001 versus DMSO group. d Cell viability of folate-deficient mESCs incubated by various concentrations of PD98059 shows no difference. e Proteins were analyzed in Folate-deficient mESCs treated by LY294002 25uM and DMSO control