Table 1.
Primer | Product size (bp) | |
---|---|---|
miR-302a |
RT primer: 5′GTCGTATCCAGTGCGTGTCGTGGA GTCGGCAATTGCACTGGATACGACTCACCAA3′ |
67 |
F: 5′GGGGTAAGTGCTTCCATGTT3′ | ||
R: 5′CAGTGCGTGTCGTGGAGT3′ | ||
U6 | RT primer: 5′CGCTTCACGAATTTGCGTGTCAT3′ | 89 |
F: 5′GCTTCGGCAGCACATATACTAAAAT3′ | ||
R: 5′CGCTTCACGAATTTGCGTGTCAT3′ | ||
AKT | F: 5′GGCTGCTCAAGAAGGACCCTAC3′ | 72 |
R: 5′GGTGCTGCATGATCTCCTTGG3′ | ||
FOXO1 | F: 5′GTACAGCGCATAGCACCA3′ | 121 |
R: 5′GCGACAGACAGAGTTCCC3′ | ||
Bim | F: 5′GTTCTGCCGCCTTTCTG 3′ | 189 |
R: 5′TTCCCCATCTGCTGCTAA 3′ | ||
miR-302a Promoter | F: 5′GTTACCCTAATCTGTGCCATCAA3′ | 284 |
R: 5′AAGACGCCGACTTTAGACCAC3′ | ||
β-actin | F: 5′TTCCAGCCTTCCTTCTTG3′ | 182 |
R: 5′GGAGCCAGAGCAGTAATC3′ |