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. 2020 Dec 7;13:169. doi: 10.1186/s13045-020-01009-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — OPN is involved in acquired EGFR-TKI resistance in NSCLC. a Expression of soluble receptors and related proteins was examined by proteome profiler array analysis in HCC827, PC9, HCC827GR and PC9 GR cells. b, c The expression level of OPN was determined by quantitative real-time PCR and western blot analysis. d Expression of secreted OPN (sOPN) was verified by ELISA in HCC827GR and PC9GR cells. e, f qRT-PCR and ELISA were used to detect expression of OPN in PC9GR cells after transfection with si-OPN. g The sensitivity of PC9GR cells transfected with si-OPN or si-NC to gefitinib was determined by CCK-8 assays. h Western blot analysis was used to detect expression of p-AKT and p-ERK in HCC827GR cells transfected with si-OPN or si-NC (**P < 0.01; ***P < 0.001)