Figure 2.

Characterization and quantities of OMVs derived from the ∆omp mutants and parental strain of Salmonella Typhimurium. (A) Protein contents in OMVs from the mutants and parental strain, analyzed by SDS-PAGE. The outer membrane proteins OmpC, OmpD, and OmpA are marked on the right. Strains (from left to right): K015 (∆ompA), K016 (∆ompC), K017 (∆ompD), K018 (∆ompA∆ompC), K019 (∆ompA∆ompD), K020 (∆ompC∆ompD), K021 (∆ompA∆ompC∆ompD), and χ3761 (parent). (B) Numbers of particles per milliliter were normalized to numbers of CFU per milliliter calculated from each mutant and parental strain. (C) Concentrations of OMVs derived from the ∆omp mutants and parental strain (same order as in A). Data are presented as means ± standard deviations (SD) for three independent cultures. ^*p < 0.05 and ^**p < 0.01.