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Sequences of primers used in the qRT-PCR
| Gene | Primer (5′ → 3′) | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|
| FOXO1 | F: TTTGCCCCAGATGCCTATAC | 114 | 57.5 |
| R: GGAGAGTCAGAAGTCAGCAAC | |||
| Bax | F: TTTCCGAGTGGCAGCTG | 74 | 55.8 |
| R: CAAAGTAGAAAAGGGCGACAAC | |||
| Bcl-2 | F: GGATGCCTTTGTGGAACTGT | 135 | 57.4 |
| R: CACTTGTGGCTCAGATAGGC | |||
| caspase-9 | F: TAACAGGCAAGCAGCAAAGT | 139 | 53.4 |
| R: ACCAAATCCTCCAGAACCAA | |||
| caspase-3 | F: AGAACTGGACTGTGGCATTG | 111 | 55.4 |
| R: TAACCAGGTGCTGTGGAGTA | |||
| GAPDH | F: ACGGATTTGGTCGTATTGGG | 214 | 57.4 |
| R: CGCTCCTGGAAGATGGTGAT |
