Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 7;8:212. doi: 10.1186/s40478-020-01075-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 1 — Generation of CLAC-P tg mice and APP/CLAC-P double tg mice. a A schematic structure of human CLAC-P (hCLAC-P) transgene using murine Thy1.2 promoter. The arrows show the location of human CLAC-P specific primers. Scale bar shows 1 kbp. b RT-PCR analyses in CLAC-P tg mice (line#74) using human CLAC-P (upper panel) and murine β-actin (lower panel) specific primers. c Immunoblot analysis of the brains of CLAC-P tg and its littermate wild-type mice (wt). Mice brains were extracted sequentially by Tris saline (cytosolic fraction, C) and 2% Triton X-100 (membrane fraction, M). Arrow shows the mobility of CLAC-P in HEK293 cells. Asterisks show the non-specific bands. d Schematic representation of the domain structure of human CLAC-P. The NC1 domain composed of cytoplasmic, transmembrane and extracellular portions, three extracellular non-collagenous domains (NC2, NC3 and NC4) and the three collagenous domains (COL1, COL2 and COL3) are shown. The epitope locations of the antibodies are shown in black bars. e Immunohistochemical analyses of cerebral cortex of 6-month-old CLAC-P tg (upper panels) or wild-type mice (lower panels) by anti-NC1, NC4, and pyroE113 human CLAC-P specific antibodies. The plaque-like structures are exclusively positive for the extracellular epitope of CLAC-P (arrows). Scale bar shows 20 μm. f, h Immunoblots of human APP and human CLAC-P in membrane fraction of the brains of double (J20 (f), A7 (h)) tg mice by anti-human APP antibody (BAN50 upper panel in f, 6E10 in h), or anti-CLAC-P antibody (anti-NC4 lower panel in (h)). g Densitometric analyses of the levels of full-length APP in the brains of APP (J20) and double tg mice. The mean ± SD N = 6 (APP), 7 (double). Student’s t-test, p = 0.89. i Densitometric analyses of the levels of full-length APP in the brains of APP (A7) and double tg mice. The mean ± SD is shown. N = 13 (APP), 9 (double). Student’s t-test, p = 0.90. j Immunoblot analysis of the APP CTFs (APP CTF-α and APP CTF-β) in the brains of APP (J20) and double tg mice by anti-APP C-terminus. k Densitometric analyses of the levels of APP CTFs (normalized by the levels of full-length APP). The mean ± SD N = 4 (APP), 3 (double) tg. Student’s t-test, p = 0.58 (APP CTF-α) and 0.71 (APP CTF-β)