Fig. 3.

Overexpression of CLAC-P altered the morphology of Aβ plaques (A7 line). a, b Immunohistochemical analyses of the entorhinal cortex of 18-month-old APP tg (A7, A) and double tg (A7 × CLAC-P, B) mice using an anti-human Aβ antibody (82E1). Scale bar shows 250 μm. c Quantitative analysis of the amyloid burden (% Aβ immunoreactive areas) in the parietal cortex, piriform cortex and frontal cortex of APP tg and double tg mice. N = 4, Student’s t-test, p = 0.069 (parietal cortex), 0.024 (piriform cortex), 0.10 (frontal cortex), * p < 0.05. d Qantitative analysis of the amyloid burden at the molecular layer of hippocampal dentate gyrus of 18-month-old APP and double tg mice. e N = 15 (APP tg) and 12 (double tg). Student’s t-test, p = 0.00061, **, p < 0.01. e, f Average circularity (e) and size (f) of Aβ plaques in the hippocampus of 18-month-old APP and double tg mice. N = 15 (APP tg) and 12 (double tg). Student’s t-test, p = 0.0061, **, p < 0.01 (f) N = 15 (APP tg) and 12 (double tg). Student’s t-test, p = 0.0046, **, p < 0.01