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. 2020 Dec 7;8:212. doi: 10.1186/s40478-020-01075-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 9 — Similar levels of insoluble Aβ in the brains of APP and double tg mice. a The concentration of Aβ40 (circles) and Aβ42 (triangles) in the 2% SDS-insoluble, 70% formic acid-soluble fraction of the brains of 12-month-old wild-type (N = 7), CLAC-P tg (N = 7), APP tg (J20 line, N = 13) and double tg (N = 14) mice were measured by a two-site ELISA. Student’s t-test, p = 0.40 (Aβ40 in APP tg vs Aβ40 in double tg), 0.44 (Aβ42 in APP tg vs Aβ42 in double tg). n.s. means no significant difference. b The levels of Aβ40 and Aβ42 in the SDS-insoluble, 70% formic acid-soluble fraction of the brains of 6-, 9-, 12- and 15-month-old double tg mice and littermates. The mean ± SD of the amount of Aβ40 and Aβ42 are shown