FIGURE 5.

Effect of BI 1265162 on a) water transport and b) MCC in normal and CF MucilAir^TM cultures. The effect of BI 1265162 with or without CFTR modulators and isoproterenol (an upregulator of CFTR expression) on water transport and MCC was evaluated in a pseudostratified, fully differentiated 3D model of human epithelium (n=5 cultures per treatment) employing bronchial cells from a CF and a non-CF donor. Water transport was measured on Days 2 and 5 of the study by weighing the membrane inserts containing the cells. MCC was measured on Day 7 by video-tracking the movements of polystyrene microbeads added to the apical surface of MucilAir™. Error bars show standard error of mean. MCC: mucociliary clearance; CF: cystic fibrosis; CFTR: cystic fibrosis transmembrane conductance regulator; ISO: isoproterenol; IVA/LUM: ivacaftor/lumacaftor. ^#: MucilAir™ culture medium + penicillin/streptomycin + 0.1% dimethyl sulfoxide. *: p<0.05; **: p<0.01; ***: p<0.001; ^¶: p<0.0001 vehicle versus BI 1265162.