Skip to main content
. 2020 Dec 7;6(12):e05672. doi: 10.1016/j.heliyon.2020.e05672

Figure 3.

Figure 3

AGER is required for HMGB1-induced ACE2 expression in lung epithelial cells. (A) ELISA analysis of TNF release in indicated human monocytes (THP1) following treatment with human HMGB1 protein (200 ng/ml) for 24 h (n = 3, t test). (B) Western blot analysis of protein expression in human lung epithelial cells (Calu-3) after siRNA-mediated depletion of TLR4 or AGER. (C) Q-PCR analysis of ACE2 mRNA in indicated Calu-3 cells following treatment with human HMGB1 protein (200 ng/ml) for 24 h (n = 3, t test). Data represent the mean ± SD of three independent experiments, each performed in triplicate, and are presented relative to control.