Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 7;326:21–27. doi: 10.1016/j.jbiotec.2020.12.005

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Crown Copyright © 2020 Published by Elsevier B.V. All rights reserved.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

PMC Copyright notice

Fig. 2 — Evaluation of mammalian cell expression platforms for expression of SARS-CoV-2 ectodomain. HEK293, CHO-3E7, or CHO^BRI/55E1 cells were transfected with the indicated percentages of ECD-coding plasmid DNA. (A) Comparison of ECD-RSTN production in HEK293 and CHO-3E7 cells. Samples of culture supernatant were taken at 5 days post-transfection (dpt). (B) Comparison of ECDm-RSTN production in CHO-3E7 and CHO^BRI/55E1 cells. Samples of culture supernatant were taken at At 5 dpt (CHO-3E7) or 6 dpt (CHO^BRI/55E1). For all samples, 10 μL of culture supernatant was analyzed by western blotting. Ponceau S staining of membranes before western blotting is shown in lower panels.