KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-AMYLASE (IHC 1:500) | Sigma | Cat #A8273 |
| Rabbit anti-CASPASE3 (IHC 1:300) | Cell Signaling | Cat #9661S |
| Rat anti-CD31 (PECAM-1) (IHC 1:100) | BD | Cat #550274 |
| Rabbit anti-C-PEPTIDE (IHC 1:250) | Cell Signaling | Cat #4593S |
| Rabbit anti-COLLAGEN I (IHC 1:500) | Abcam | Cat #ab34710 |
| Rabbit anti-DESMIN (IHC 1:100) | Cell Signaling | Cat #5332S |
| Rat anti-E-CADHERIN (IHC 1:1000) | Invitrogen | Cat #U3254 |
| Rat anti-F4/80 (IHC 1:100) | BioLegend | Cat #BLD 123101 |
| Rabbit anti-FIBRONECTIN (IHC 1:1000) | gift of Prof. K M. Yamada (NIH, USA) | N/A |
| Chicken anti-GFP (IHC 1:400) | Aves Labs | Cat #GFP-1020 |
| Rabbit anti-GLUCAGON (IHC 1:500) | Immunostar Inc. | Cat #20076 |
| Goat anti-GLUT2 (IHC 1:200) | Santa Cruz | Cat #sc-7580 |
| Guinea pig anti-INSULIN (IHC 1:400) | Invitrogen | Cat #PA1-26938 |
| Rat anti-INTEGRIN α6 (CD49f, VLA-6) (IHC 1:400) | Millipore | Cat #MAB1378 |
| Mouse anti-ISLET-1 (IHC 1:100) | Hybridoma Bank | Cat #39.4D5 |
| Rabbit anti-LAMININ (IHC 1:1000) | Sigma | Cat #L9393 |
| Rabbit anti-MAFA (IHC 1:500) | Abcam | Cat #ab26405 |
| Armenian hamster anti-MUCIN (IHC 1:500) | Thermo Scientific | Cat #HM-1630-P1 |
| Guinea pig anti-NEUROGENIN3 (IHC 1:400) | Millipore | Cat #AB10536 |
| Goat anti-NEUROGENIN3 (IHC 1:1200) | BCBC | Cat #AB2774 |
| Rabbit anti-NEUROD1 (IHC 1:800) | Abcam | Cat #ab213725 |
| Mouse anti-NKX6.1 (IHC 1:250) | Hybridoma Bank | Cat #F55A10 |
| Rabbit anti-PAX6 (IHC 1:500) | Covance | Cat #PRB-278P |
| Guinea pig anti-PDX1 (IHC 1:500) | Abcam | Cat #ab47308 |
| Rabbit anti-PDGFRb (IHC 1:500) | Abcam | Cat #ab32570 |
| Mouse anti-PHOSPHO-HISTONE H3 (Ser10) (IHC 1:100) | Cell Signaling | Cat #9706 |
| Rabbit anti-PHOSPHO-HISTONE H3 (Ser10) (IHC 1:200) | Millipore | Cat #06-570 |
| Rabbit anti-PBX1 (IHC 1:400) | Cell Signaling | Cat #4342 |
| Rabbit anti-PROX1 (IHC 1:200) | RELIATech GmbH | Cat #102-PA32S |
| RAT anti-RFP (IHC 1:400) | Chromotek | Cat #ABIN334653 |
| Rabbit anti-VIMENTIN (IHC 1:100) | Cell Signaling | Cat #5741S |
| Goat Alexa Fluor 488-labeled Anti-Chicken IgG (IHC 1:750) | Invitrogen | Cat #A11039 |
| Donkey Alexa Fluor 488-labeled Anti-Goat IgG (IHC 1:750) | Invitrogen | Cat #A11055 |
| Donkey Alexa Fluor 594-labeled Anti-Goat IgG (IHC 1:750) | Invitrogen | Cat #A11058 |
| Donkey Alexa Fluor 488-labeled Anti-Guinea Pig IgG (IHC 1:750) | Dianova | Cat #706-545-148 |
| Donkey Alexa Fluor 647-labeled Anti-Guinea Pig IgG (IHC 1:750) | Dianova | Cat #706-605-148 |
| Goat DyLight 649-labeled Anti-Armenian Hamster IgG (IHC 1:750) | Dianova | Cat #127-495-099 |
| Donkey Alexa Fluor 594-labeled Anti-Mouse IgG (IHC 1:750) | Invitrogen | Cat #A21203 |
| Donkey Alexa Fluor 647-labeled Anti-Mouse IgG (IHC 1:750) | Invitrogen | Cat #A31571 |
| Donkey Alexa Fluor 488-labeled Anti-Rabbit IgG (IHC 1:750) | Invitrogen | Cat #A21206 |
| Donkey Alexa Fluor 594-labeled Anti-Rabbit IgG (IHC 1:750) | Invitrogen | Cat #A21207 |
| Donkey Alexa Fluor 647-labeled Anti-Rabbit IgG (IHC 1:750) | Invitrogen | Cat #A31573 |
| Donkey Alexa Fluor 488-labeled Anti-Rat IgG (IHC 1:750) | Invitrogen | Cat #A21208 |
| Donkey Alexa Fluor 594-labeled Anti-Rat IgG (IHC 1:750) | Invitrogen | Cat #A21209 |
| Donkey Alexa Fluor 647-labeled Anti-Rat IgG (IHC 1:750) | Dianova | Cat #712-605-153 |
| Biological Samples | ||
| Gastrointestinal tract dissected from mouse embryos and adults at different stages | This paper | N/A |
| Pancreatic explants from E12.5 mouse embryos | This paper | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| ALK5 Inhibitor II | Enzo Life Sciences | Cat #ALX-270-445-M001 |
| CTSTM B-27® Supplement, XenoFree (100X) | ThermoFisher Scientific | Cat #A1486701 |
| Hoechst 33342 (250 ng/mL) | Invitrogen | Cat #H1399 |
| HCS Cell Mask Blue (2μg/mL) | Invitrogen | Cat #H32720 |
| ITS, Insulin-Transferrin-Selenium Supplement (100X) | ThermoFisher Scientific | Cat #41400-045 |
| LDN-193189 BMP inhibitor | Tebu-Bio | Cat #24804-0074 |
| Recombinant Human EGF | R&D Systems | Cat #236-EG-200 |
| Recombinant Human Heregulinβ-1 | Peprotech | Cat #100-03-10 |
| Recombinant Human KGF/FGF-7 Protein | R&D Systems | Cat #251-KG-050 |
| Recombinant Human/Mouse/Rat Activin A Protein, CF | R&D Systems | Cat #338-AC-050/CF |
| Recombinant Human Slit3 Protein, CF | R&D Systems | Cat #9067-SL-050 |
| Recombinant Human Wnt-3a Protein, CF | R&D Systems | Cat #5036-WN-010/CF |
| Recombinant Mouse Slit1 Protein, CF | R&D Systems | Cat #5199-SL-050 |
| Recombinant Mouse Slit3 Protein, CF | R&D Systems | Cat #9296-SL-050 |
| Rock Inhibitor Y-27632 | Sigma | Cat #SCM075 |
| TBP, α-Amyloid Precursor Protein Modulator - CAS 497259-23-1 - Calbiochem | Millipore | Cat #565740-1MG |
| TGF-β RI Kinase Inhibitor IV | Millipore UK Ltd | Cat #616454-2MG |
| TTNPB, stable retinoid analog | Sigma | Cat #T3757-10MG |
| Critical Commercial Assays | ||
| FastStart Essential DNA Green Master Mix | Roche | Cat #06924204001 |
| High Pure RNA Isolation Kit | Roche | Cat #11828665001 |
| Human C-peptide ELISA kit | Mercodia | Cat #10-1141-01 |
| Human Insulin ELISA kit | Mercodia | Cat #10-1132-01 |
| RNAscope Multiplex Fluorescent Reagent Kit v2 | Advanced Cell Diagnostics | various |
| Radioimmunoassay kit | Merck-Millipore | Cat #RI-13K |
| SYBRGreen Master Mix | Sigma Aldrich Co Ltd | Cat #4913914001 |
| Transcriptor First Strand cDNA Synthesis Kit | Roche | Cat #04379012001 |
| TUNEL Apo-Green Detection Kit | Biotool | Cat #B31115 |
| Deposited Data | ||
| RNAseq data from mouse pancreatic epithelial and mesenchymal populations | This paper | GEO accession number: GSE123759 |
| Transcriptome from human fetal pancreas | Ramond et al., 2017 | GEO accession number: GSE96697 |
| Experimental Models: Cell Lines | ||
| BIHi043-A (XM001) | Wang et al., 2018 | N/A |
| BIHi005-A | Berlin Institute of Health, Germany | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: Nxk2.5-Cre | Stanley et al., 2002 | Nkx2-5tm2(cre)Rph |
| Mouse: Nxk3.2-Cre | Verzi et al., 2009 | Nkx3-2tm1(cre)We |
| Mouse: Pdx1-Cre | Hingorani et al., 2003 | B6.FVB-Tg(Ipf1-cre)1Tuv |
| Mouse: Pbx1flox/flox | Koss et al., 2012 | Pbx1tm2Mlc |
| Mouse: Pbx2 −/− | Selleri et al., 2004 | Pbx2tm1Mlc |
| Mouse: R26R-mTmG | Muzumdar et al., 2007 | B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J |
| Oligonucleotides | ||
| Antisense I Slit3 for ISH | Gift of F. Bareyre (LMU, Munich, Germany) | N/A |
| Human primers for RT-qPCR | This paper | See Table S2 |
| Mouse primers for RT-qPCR | This paper | See Table S2 |
| RNAscope Probe-Mm-Nkx3-2-C1 | Advanced Cell Diagnostics | Cat #526401 |
| RNAscope Probe-Mm- Nkx2-5-C2 | Advanced Cell Diagnostics | Cat #428241-C2 |
| Software and Algorithms | ||
| Cufflinks (2.2.1) | Trapnell lab | RRID:SCR_014597 |
| DAVID GO analysis | Huang et al., 2009 | RRID:SCR_001881 |
| GraphPad Prism 7 | GraphPad | RRID:SCR_002798 |
| ImageJ 1.53 | ImageJ | RRID:SCR_003070 |
| Imaris version 9.5 | BitPlane | RRID:SCR_007370 |
| R | N/A | RRID:SCR_001905 |
| Star 2.4.0j | Dobin et al., 2013 | N/A |
| Zen Digital Imaging for Light Microscopy | Carl Zeiss AG | RRID:SCR_013672 |
| Huygens software (SVI) | SVI | RRID:SCR_014237 |