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. Author manuscript; available in PMC: 2021 Nov 19.
Published in final edited form as: Mol Cell. 2020 Nov 19;80(4):633–647.e7. doi: 10.1016/j.molcel.2020.10.026

Figure 4. Phosphorylation of METTL3/WTAP by ERK Facilitates Resolution of Pluripotency.

Figure 4.

(A) LC-MS/MS quantification of the m6A/A ratio in mRNA of mESCs stable transfectants. *p < 0.05, **p < 0.01, ***p < 0.001.

(B) Cell growth of R-WT and R-3A2A mESCs were measured by sulforhodamine B dye (SRB assay). Data are presented as relative to Day 1. ***p < 0.001.

(C) MeRIP-qPCR of pluripotency transcripts in mESCs stable transfectants. *p < 0.05, **p < 0.01.

(D) qPCR analysis of pluripotency genes in mESCs stable transfectants. *p < 0.05, **p < 0.01. (E) qPCR analysis for pluripotency and differentiation markers expression after 8 days of embryonic body induction. *p < 0.05, **p < 0.01, ***p < 0.001.

Unless otherwise indicated, all data in this figure contain n = 3 replicates per group, and represent mean ± SEM. All p-values were also calculated by Student’s t test.

See also Figure S5.