Figure 5. Transcripts Affected by Phosphorylation of Methyltransferase Complex in mESCs.

(A) Cumulative distribution function of log2 peak intensity of m⁶A-modified sites in R-WT and R-3A2A mESCs.

(B) Volcano plot for peaks with differential m⁶A intensity between R-WT and R-3A2A mESCs. Fold change (FC) is the ratio of IP over Input for R-WT and R-3A2A.

(C) Coverage plots of the m⁶A peaks of Nanog, Lefty1, and Zfp219 comparing R-WT and R-3A2A mESCs. Plotted coverages are the medians of three replicates.

(D) Gene enrichment analysis with WikiPathway terms of differentially m⁶A methylated peaks in R-WT and R-3A2A mESCs for molecular functions.

(E) Gene enrichment analysis with WikiPathway terms of differentially expressed genes (p < 0.05).

(F) A histogram showing relative m⁶A peak enrichment of R-3A2A compared to R-WT mESCs, indicating higher m⁶A methylation in pluripotency genes (PluriNetwork) for R-WT mESCs.

See also Figure S6, Table S3, Table S4, and Table S5.