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. 2020 Dec 10;77(1):147–157. doi: 10.1161/HYPERTENSIONAHA.120.16002

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© 2020 The Authors.

Hypertension is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 3. — Changes in HIF-1α (hypoxia-inducible factor-1α) after Ang II. A, HIF-1α mRNA expression relative to rpl19 (ribosomal protein L 19) measured by real-time PCR in the paraventricular nucleus of the hypothalamus (PVN) and (B) cortex of vehicle and Ang II (angiotensin II)-infused group. The 2^−ΔΔCT method was used to calculate relative changes in gene expression, which relates the expression of the target gene to the expression of a reference gene. Changes in HIF-1α mRNA are presented in folds with reference to the Saline group. C, Protein expression of HIF-1α expression in the PVN and (D) cortex of saline and Ang II-infused group top: a representative Western blot, bottom: densitometric analyses of HIF-1α level normalized to tubulin. E, Immunofluorescent staining of PVN sections from saline and Ang II-infused rats. HIF-1α (green) and nuclei (blue). F, Average values obtained by the reciprocal intensity method in each group were shown. Values are mean±SEM of analyses of 4 to 6 animals in each group. T-test (with Welch correction [A–D], and [F]). *P=0.010 (A); P=0.0721 (B); **P=0.004 (C); P=0.0626 (D); *P=0.016 (F) vs saline control.