Fig. 2.
Mid-IR PAM imaging of a mouse brain slice. Mid-IR PAM imaging of a mouse brain slice. (a) A photo of the thick manually cut brain slice. (b) PA spectra measured at the two sites on the brain slice indicated by the acronyms in (a). The PA spectrum of the sphingomyelin solution (black) is also plotted here. cc, corpus callosum (blue) and hc, hippocampus (red). (c) PAM image of the region of interest denoted by the dashed box in (a). alv, alveus; fr, fasciculus retroflexus; lv, lateral ventricle; ml, medial lemniscus; and pag, periaqueductal gray nucleus. Scale bar, 1 mm. (d) Enlarged view of the retrosplenial area [red box in (c)]. (e) Enlarged view of the superior cerebellar peduncle [yellow box in (c)]. Scale bars in (d) and (e), .