Figure 4. SpEDIT shows reduced toxicity compared with the current pMZ379 S. pombe CRISPR/Cas9 system in asynchronous cells.

A– B. Colony area measurements of asynchronous ( A) or G1-synchronized ( B) wild-type cells transformed with pLSB or pMZ379 plasmids and indicated HR templates growing on selective cloNAT-containing plates (same experiment as Figure 3). Colony area was quantified (cm ²) using ImageJ. sgRNA, single guide RNA. HR, homologous recombination donor template.